Ribonuclease (RNase) protection is a common and powerful technique used for the analysis of RNA levels. RNA probes used in RNase protection are commonly produced by in vitro transcription of DNA ...

This is a preview. Log in through your library . Abstract Polymerase chain reaction (PCR) was used to produce biotinylated DNA probes for human papillomavirus (HPV) types 16 and 18. The specificity ...

New probes using incredibly bright dyes could help detect COVID-19 earlier, leading to faster testing even when only trace amounts of SARS-CoV-2 RNA are present. As the COVID-19 pandemic continues, ...

image: Eprobe®, a highly efficient and reliable fluorescent probe for PCR DNA amplification techniques and DNA analysis in hybridization experiments, has been developed by researchers from RIKEN and ...

Real-time PCR or quantitative PCR (Polymerase Chain Reaction) is an advancement of the standard PCR technique, which enables continuous monitoring of the reaction and collection of data in real time.

The most common molecular assays rely on identifying the viral genomic material from a clinical specimen. In general, there are three important steps to this process that must be addressed for an ...

PCR genetic analysis has been in the spotlight since COVID-19, but light is now further facilitating PCR-free methods. Osaka Metropolitan University scientists have developed a light-induced DNA ...

In its formative years PCR and its intellectual possessors had to go through some legal wrangling over the originality of the technique. In point of fact, a 13-year earlier paper by Nobelist Gobind ...

High-resolution melting has proven to be a highly sensitive method for mutation discovery. PCR is performed in the presence of the double-strand DNA binding dye LCGreen® Plus. Samples are then ...

Taqman hybridization probes have been used for mutant allele detection by DNA melting curve analysis. Now researchers have also cleverly used these probes in another capacity in the assay in order to ...

As SARS-C0V-2 variants emerged, researchers developed a PCR test that uses molecular beacons not only to diagnose COVID-19 infection, but also to identify the specific variant causing that infection.